Thiocyanate hydrolase
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Article ImagesA thiocyanate hydrolase (EC 3.5.5.8) is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase. This enzyme catalyzes the chemical reaction:
thiocyanate hydrolase | |||||||||
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Identifiers | |||||||||
EC no. | 3.5.5.8 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
Gene Ontology | AmiGO / QuickGO | ||||||||
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- SCN− + 2 H2O + H+ ⇌ SCO + NH3
The mechanism is proposed to involve a metal thiocyanate complex.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2DD4, 2DD5, 2DXB, and 2DXC.
A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate:[1]
- SCN− + H2O → OCN− + H2S
- ^ Tikhonova, Tamara V.; Sorokin, Dimitry Y.; Hagen, Wilfred R.; Khrenova, Maria G.; Muyzer, Gerard; Rakitina, Tatiana V.; Shabalin, Ivan G.; Trofimov, Anton A.; Tsallagov, Stanislav I.; Popov, Vladimir O. (2020). "Trinuclear Copper Biocatalytic Center Forms an Active Site of Thiocyanate Dehydrogenase". Proceedings of the National Academy of Sciences. 117 (10): 5280–5290. Bibcode:2020PNAS..117.5280T. doi:10.1073/pnas.1922133117. PMC 7071890. PMID 32094184.
- Katayama Y, Matsushita Y, Kaneko M, Kondo M, Mizuno T, Nyunoya H (1998). "Cloning of genes coding for the three subunits of thiocyanate hydrolase of Thiobacillus thioparus THI 115 and their evolutionary relationships to nitrile hydratase". J. Bacteriol. 180 (10): 2583–9. doi:10.1128/JB.180.10.2583-2589.1998. PMC 107207. PMID 9573140.
- Katayama Y, Narahara Y, Inoue Y, Amano F, Kanagawa T, Kuraishi H (1992). "A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate". J. Biol. Chem. 267 (13): 9170–5. doi:10.1016/S0021-9258(19)50404-5. PMID 1577754.