A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : II. A STUDY OF AN AZOPROTEIN-ANTIBODY SYSTEM

J Exp Med. 1935 Sep 30; 62(4): 467–483.

Abstract

1. A quantitative theory of the precipitin reaction based on the laws of classical chemistry has been tested on an azoprotein-antiprotein system and found to apply. 2. With its aid relationships may be deduced which permit the calculation of the behavior of an antidye serum over its entire range after a few quantitative chemical analyses have been made for antigen and antibody in the precipitate. 3. An empirical relation is also presented which further reduces the number of analyses necessary. 4. A study of supernatants in the inhibition zone has shown that the entire amount of dissolved antigen-antibody compound present is precipitated when supernatants are analyzed for antigen by the precipitin method.

• Heidelberger M, Kendall FE. A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODY. J Exp Med. 1929 Nov 30;50(6):809–823. [PMC free article] [PubMed] [Google Scholar]
• Heidelberger M, Kendall FE, Soo Hoo CM. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : ANTIBODY PRODUCTION IN RABBITS INJECTED WITH AN AZO PROTEIN. J Exp Med. 1933 Jul 31;58(2):137–152. [PMC free article] [PubMed] [Google Scholar]
• Heidelberger M, Kendall FE. SPECIAL ARTICLES. Science. 1930 Sep 5;72(1862):252–253. [PubMed] [Google Scholar]
• Heidelberger M, Kendall FE. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : THE DETERMINATION OF SMALL AMOUNTS OF A SPECIFIC POLYSACCHARIDE. J Exp Med. 1932 Mar 31;55(4):555–561. [PMC free article] [PubMed] [Google Scholar]
• Hopkins FG. On the separation of a pure albumin from egg-white. J Physiol. 1900 Apr 24;25(4):306–330. [PMC free article] [PubMed] [Google Scholar]
• Heidelberger M, Kendall FE. THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND HOMOLOGOUS ANTIBODY : II. CONDITIONS FOR QUANTITATIVE PRECIPITATION OF ANTIBODY IN HORSE SERA. J Exp Med. 1935 Mar 31;61(4):559–562. [PMC free article] [PubMed] [Google Scholar]