Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis - PubMed
Tehmina Mustafa 1 ,
Article Images. 2006 Dec;19(12):1606-14.
doi: 10.1038/modpathol.3800697. Epub 2006 Sep 15.
Affiliations
- PMID: 16980944
- DOI: 10.1038/modpathol.3800697
Free article
Immunohistochemistry using a Mycobacterium tuberculosis complex specific antibody for improved diagnosis of tuberculous lymphadenitis
Tehmina Mustafa et al. Mod Pathol. 2006 Dec.
Free article
Abstract
The clinical and histological criteria used to diagnose lymphadenitis caused by Mycobacterium tuberculosis complex organisms have poor specificity. Acid-fast staining and culture has low sensitivity and specificity. We report a novel method for diagnosis of tuberculosis that uses immunohistochemistry to detect the secreted mycobacterial antigen MPT64 on formalin-fixed tissue biopsies. This antigen has not been detected in non-tuberculous mycobacteria. Polymerase chain reaction (PCR) for amplification of IS6110 from DNA obtained from the biopsies was used as a gold standard. Fifty-five cases of granulomatous lymphadenitis with histologically suspected tuberculosis obtained from Norway and Tanzania were evaluated. Four known tuberculosis cases were used as positive controls, and 16 biopsies (12 foreign body granulomas and four other non-granulomatous cases) as negative controls. With immunohistochemistry, 64% (35/55) and with PCR, 60% (33/55) of granulomatous lymphadenitis cases were positive. Using PCR as the gold standard, the classical tuberculosis histology had sensitivity, specificity, positive and negative predictive values of 92, 37, 60, and 81%, respectively, and immunohistochemistry had sensitivity, specificity, positive and negative predictive values of 90, 83, 86, and 88%, respectively. The observed agreement between PCR and immunohistochemistry was 87% (kappa = 0.73). Immunohistochemistry with anti-MPT64 antiserum is a rapid, sensitive, and specific method for establishing an etiological diagnosis of tuberculosis in histologic specimens. Immunohistochemistry has the advantages over PCR of being robust and cheap, and it can easily be used in a routine laboratory.
Similar articles
-
Mustafa T, Wiker HG, Mørkve O, Sviland L. Mustafa T, et al. Clin Exp Immunol. 2007 Oct;150(1):105-13. doi: 10.1111/j.1365-2249.2007.03476.x. Epub 2007 Aug 17. Clin Exp Immunol. 2007. PMID: 17711491 Free PMC article.
-
Tadele A, Beyene D, Hussein J, Gemechu T, Birhanu A, Mustafa T, Tsegaye A, Aseffa A, Sviland L. Tadele A, et al. BMC Infect Dis. 2014 Nov 25;14:585. doi: 10.1186/s12879-014-0585-1. BMC Infect Dis. 2014. PMID: 25421972 Free PMC article.
-
Mustafa T, Leversen NA, Sviland L, Wiker HG. Mustafa T, et al. BMC Infect Dis. 2014 Oct 3;14:535. doi: 10.1186/1471-2334-14-535. BMC Infect Dis. 2014. PMID: 25284264 Free PMC article.
-
Singh KK, Muralidhar M, Kumar A, Chattopadhyaya TK, Kapila K, Singh MK, Sharma SK, Jain NK, Tyagi JS. Singh KK, et al. J Clin Pathol. 2000 May;53(5):355-61. doi: 10.1136/jcp.53.5.355. J Clin Pathol. 2000. PMID: 10889817 Free PMC article.
-
Goel MM, Ranjan V, Dhole TN, Srivastava AN, Mehrotra A, Kushwaha MR, Jain A. Goel MM, et al. Acta Cytol. 2001 May-Jun;45(3):333-40. doi: 10.1159/000327627. Acta Cytol. 2001. PMID: 11393063 Review.
Cited by
-
Mohan MR, Sukumar KN, Amith A. Mohan MR, et al. Thorac Res Pract. 2024 May 3;25(4):158-61. doi: 10.5152/ThoracResPract.2024.23087. Online ahead of print. Thorac Res Pract. 2024. PMID: 38705986 Free PMC article.
-
Diagnostic value of tuberculosis-specific antigens ESAT-6 and CFP10 in lymph node tuberculosis.
Chen X, Duan S, Zhou X, Fang S, Gu G, Sun J, Liu F. Chen X, et al. Heliyon. 2024 Apr 5;10(8):e29251. doi: 10.1016/j.heliyon.2024.e29251. eCollection 2024 Apr 30. Heliyon. 2024. PMID: 38638946 Free PMC article.
-
Helle OMB, Kanthali M, Ishtiaq S, Ambreen A, Purohit MR, Mustafa T. Helle OMB, et al. J Pathol Clin Res. 2024 May;10(3):e12373. doi: 10.1002/2056-4538.12373. J Pathol Clin Res. 2024. PMID: 38572528 Free PMC article.
-
Carrisoza-Urbina J, Bedolla-Alva MA, Hernández-Pando R, López-Macías C, Huerta-Yepez S, Baay-Guzmán G, Juárez-Ramírez M, Gutiérrez-Pabello JA. Carrisoza-Urbina J, et al. Front Vet Sci. 2023 Apr 27;10:1105716. doi: 10.3389/fvets.2023.1105716. eCollection 2023. Front Vet Sci. 2023. PMID: 37180066 Free PMC article.
-
Nasare AS, Swain M, Roa R. Nasare AS, et al. Indian J Med Res. 2023 Jan;157(1):81-86. doi: 10.4103/ijmr.IJMR_2239_19. Indian J Med Res. 2023. PMID: 37040231 Free PMC article.